01654naa a2200241 a 450000100080000000500110000800800410001910000110006024501090007126000090018030000180018949000080020752009730021565000270118865000120121565300480122765300280127565300100130365300120131370000180132570000180134377300510136110798071999-05-14       1996    bl ---       0--  u    #d1 aLU, A.  aSignal sequence and promoter effects on the efficacy of toxin-expressing baculoviruses as biopesticides.  c1996  ap.320-332 v.7  vv.7  aBaculovirus recombinants expressing a neurotoxin gene, tox34, from the straw itch mite Pyemotes tritici have been previously shown to paralyze or kill insects approximately 50% faster than wild-type. We constructed a series of recombinants of the baculovirus Autographa californica nucleopolyhedrovirus which expressed tox34 with different signal sequences or were controlled by different promoters to evaluate their influence on toxin expression in cell culture and in insects. Heterologous signal sequences provided no significant increase in the overall levels of the mature tox34 gene product, Tox34, secreted into the tissue culture media from infected cells and no improvement in the time required for paralysis of insect hosts. The time required for paralysis was promoter-dependent; the late 6.9K DNA binding protein gene promoter was generally the most effective promoter, although an insect HSP70 promoter was equally or more effective in one of the species.  aAutographa californica  aInsecta  aAutographa californica nucleopolyhedrovirus  aInsect-selective toxins  aTox34  aToxinas1 aSESHAGIRI, S.1 aMILLER, L. K.  tBiological Controlgv.7, n.3, p.320-332, 1996.