03077naa a2200253 a 450000100080000000500110000800800410001910000170006024500910007726000090016852023790017765000200255665000110257665000120258765000200259965000140261965000130263365300210264665300250266770000160269270000170270870000260272577300720275117886091996-05-24       1988    bl ---       0--  u    #d1 aKLEIN, T. A.  aSporogony, development and ultrastrucuture of Plamodium floridense in Culex erraticus.  c1988  aThe sporogonic stages of Plamodium floridense were studied for the first time by light and electron microscopy in the midgut and salivary glands of Culex erraticus. The sporogonic cycle of P. floridense was variable, but was usually completed by 11-14 days, at 22C, after mosquitoes took an infective blood-meal. Melanization of oocysts and degeneration of sporozoites occasionally occurred and may have contributed to the variability in time when sporozoites were observed in the salivary glands. Sporozoites were in the range of 8-22 x 0.8-1.5 um and were approximately three times the lenght of sporozoites of two other spe- cies of saurian malaria, Plamodiummexicanum and Plamodium agamae. Oocytes were usually extracellular, extending into the hemocoel. However, some also protruded into the lumen of the midgut. early, non-vacuolated oocysts had translucent lipid-like droplets, mito- chondria, large nuclei with dense nucleoli, and thre types of inclusion bodies: circular gra- nules of moderate density, more dense granules associated with an irregular space of low density, and lamellar  forms associated with an irregular space of low density. Differentia- tion of the oocyst began with internal vacuolization of the sporoblastoid, which later sub- divided into several residual bodies. Linear extensions of the internal vacuoles extended to the surface of the sporoblastoid to form subcapsular spaces. Sporozoite formations was initiated by the formation of a pair of convex electron dense membranes along the lenght of the vacuolar extensions. One or more moderately electron-dense inclusion bodies, a nucleus, and a mitochndrion migrated into the evaginating sporozoites. Rhoptries and mi- cronemes formed as the moderately dense inclusion bodies degenerated prior to the for- mation of mature sporozoites. Sporozoites were released from the oocysts and then ap- peared in the salivary of C. erraticus. Sporozoites were intracellular and were observed in groups within a vacuolar space in the salivary gland cell. Ten or 11 subpellicular microtu- bules were arranged asymmetrically in the anterior third of sporozoites within oocysts, two lying dorsolaterally or laterally to the remaining eight or nine arranged along the ventral sur- face. Microtubules within salivary gland sprozoites were rarely seen, but were clumped together in small groups when visible.  aCulex erraticus  amidgut  aoocysts  asalivary glands  asporogony  aMosquito  aGlandula salival  aPlamodium floridense1 aAKIN, D. C.1 aYOUNG, D. G.1 aTELFORD JUNIOR, S. R.  tInternational Journal for Parasitologygv.18, n.6, p.711-719, 1988.