01583naa a2200265 a 450000100080000000500110000800800410001910000170006024500920007726000090016952008680017865000180104665000200106465000180108465000180110265000130112065000220113365300210115565300150117665300190119165300130121065300120122370000210123577300610125617853111995-08-04       1977    bl ---       0--  u    #d1 aSAYRE, K. D.  aComparison of types of fermentation vessels for an in vitro artificial rumen procedure.  c1977  aThree types of containers (125-ml Erlenmeyer flasks 122 x 28 mm glass centriguge tubes, and 200 x 25 mm glass screwcapped tubes) were compared as fermentation vessels for modified Tilley and Terry two-stages in vitro artificial rumen procedure. This in vitro technique estimates true dry matter digestibility. Four forages (orchard-grass, alfalfa, bromegrass, and timothy) with wide differences in digestibility and three sample sizes (250, 375, and 500 mg) were utilized for the comparison. Digestibilities were consistently low with the centrifuge tubes. Results with screwcapped tubes were comparable to those with Erlenmeyer flasks. Tubes utilize water bath space more efficiently and do not require a constant supply of CO2 during fermentation to maintain an anaerobic condition. Small but significant differences in digestibly occurred between sample sizes.  adigestibility  aDigestibilidade  aFermentação  aMatéria Seca  aPastagem  aRúmen Artificial  aArtificial rumen  aDry matter  aFermen- tation  aIn vitro  aPasture1 aSOEST, P. J. van  tJournal of Dairy Sciencegv.55, n.10, p.1496-1498, 1977.