02107naa a2200229 a 450000100080000000500110000800800410001910000210006024501480008126000090022952013980023865300250163665300220166165300080168370000210169170000170171270000210172970000210175070000140177170000290178577300630181417851172010-03-03       2007    bl ---       0--  u    #d1 aTOMICH, R. G. P.  aLeptospirosis serosurvey in bovines from Brazilian Pantanal using igg elisa with recombinant protein lipl32 and microscopic agglutination test.  c2007  aThis investigation was carried out in Brazilian Pantanal: region with important biodiversity. This region's climatic conditions, hydrology and geomorphology as well as the existence of great variety of wild species favor the maintenance of the Leptospira in the environment. The aim of this study was to evaluate IgG ELISAwith recombinante protein LipL32 in comparison with microscopic agglutination test (MAT) and additionally contribute to the knowledge of the distribution of the one of most important wordwide zoonotic infection, assessing the seropositivity of bovine leptospirosis in beef cattle herds of Brazilian Pantanal, an importantecological preserved area, where cattle constitute not only the most important economic resource but also the major activity compatible of the conservation of natural resource of the region. Out of 282 samples of cattle serum analyzed, 143 (50.71%) were positive in MAT. The serovar Hardjo (genotypic Hardjoprajitno and Hardjobovis), Wolffi and Ballum showed the largest frequency of reactive samplas. In the IgG ELISA ArLipL32, 161 samples (57.09%) were positive. This result was higher than obtained by MAT (p&lt0,001). The sensitivity of the ELISA test was 99.30% and the specificity was 86.33%, based on the MAT. This test was shown to be a more sensitive, specific and accurate test for the diagnosis of bovine leptospirosis compared to the MAT.  aBovine Leptospirosis  aIgG ELISA rLipL32  aMAT1 aBOMFIM, M. R. Q.1 aKOURY, M. C.1 aPELLEGRIN, A. O.1 aPELLEGRIN, L. A.1 aKO, A. I.1 aBARBOSA-STANCIOLI, E. F.  tBrazilian Journal of Microbiologygn. 38, p. 674-680, 2007