01913naa a2200229 a 450000100080000000500110000800800410001910000210006024501430008126000090022452012010023365000230143465000090145765000150146665000090148165300230149065300360151365300140154965300210156370000160158477300830160017849931995-07-26       1978    bl ---       0--  u    #d1 aHOLTZCLAW, K. M.  aAnalytical properties of the solube, metal-complexing fractions in sludge-soil mixturesbIII.Unaltered anionic surfactants in fulvie acid.  c1978  aA rapid analytical procedure was developed for the quantititative determination of unbiodegraded anionic surfactants in the fulvic acid fraction of sewage sludge. This procedure, which is a modification of the methyl green method of Moore and Kolbeson, consists of: (i) hydrolysis in 4N HCl, (ii) separation on an anion exchange resin, (iii) extraction of a methyl green-surfactant complex into benzene, and (iv) colorimetric determination of the complex. The modified procedure is free of the nehative interferences that occurred when the original methyl green method was applied to a sludge-derived fulvic acid and does not require specialized reagents or expensive instrumentation. An application of the modified procedure to two representative fulvic acids extracted from anerobically-digested sewage sludges indicted that about 5% of the dry mass of a sludge-derived fulvic acid (water- and ash-free basis) may be attributed to unaltered anionic surfactant compounds. This result suggeste that a significant aspect of the chemistry of agricultural soils into which sewage sludge has been incorporated will be reactions involving both unaltered and partially biodegraded anionic surafactants.  aanalytical methods  asoil  aDetergente  aSolo  aBiodegradabilidade  aBiodegradability of surfactants  aDetergent  aMetodo analitico1 aSPOSITO, G.  tSoil Science Society of America Journalgv.42, n.4, p.607-610, July/Aug. 1978.