01786naa a2200229 a 450000100080000000500110000800800410001910000210006024501010008126000090018252011660019165000110135765000110136865300110137970000200139070000170141070000140142770000180144170000150145970000140147477300680148817837372017-04-06       1990    bl ---       0--  u    #d1 aGILLESPIE, J. H.  aComparison of persistence of seven bovine viruses on bovine embryos following in vitro exposure.  c1990  aThe ability of seven cytopathic strains of bovine viruses to adhere to the zona pellucida of six-to-eight day-old bovine embryos were compared. Embryos were exposed to virus by placing them either in virus suspensions or by culturing them on infected bovine turbinate cultures for 18-24 h. After exposure to bovine virus diarrhea virus (BVDV), infectious bovine rhinotracheitis virus (BTV), pseudorables virus (PRV), vesicular stomatitis virus (VSV), parainfluenza 3 virus (PI3), or bovine enterovirus virus (BEV), the embryos were tested for virus by culture in bovine turbinate cells and by morphological examination using electron microscopy (EM). A special technique to minimize loss embryos processed for EM was developed. More embryos had viral particles on the surface of the zona pellucida after exposure to 18-24 hour infected cell cultures than did embryos exposed to viral culture suspension. The most dramatic finding was that BTV adhered in large numbers to the surface of the zona pellucida of exposed embryos, IBRV, PRV and VSV comprised an intermediate group, with virions occasionally detected on the surface of exposure embryos after 5 washes.  aBovino  aVírus  aBovine1 aSCHLAFER, D. H.1 aFOOTE, R. H.1 aQUICK, S.1 aDOUGHERTY, E.1 aSCHIFF, E.1 aALLEN, S.  tDeutsche Tierarztliche Wochenschriftgv.97, n.2, p.65-68, 1990.