02283naa a2200325 a 450000100080000000500110000800800410001910000170006024501110007726000090018830000160019752013320021365000170154565000200156265000220158265000120160465000110161665000310162765000220165865000200168065000230170065000250172365300190174870000150176770000130178270000160179570000150181170000150182677301160184116683702019-01-18 1999 bl uuuu u00u1 u #d1 aBARCELOS, E. aMolecular markers applied to the analysis of genetic diversity and to the biogeography of Elaeis (Palmae). c1999 ap. 191-201. aGenetic diversity in the genus Elaeis was evaluated by the restriction fragment length polymorphism (RFLP) and amplified fragment length polymorphism (AFLPtm) techniques, on a set of accessions from E. oleifera (241 accessions), E. guineensis (39), and Barcella odora (three accessions), representing the geographical areas of distribution for the genus. RFLPs were detected by hybridizing 13 nuclear and four mitochondrial probes onto blots of total DNA extracted from individuals and digested by one of the following restriction enzymes: EcoRI, Bg/II, SstI, or EcoRV. AFLP was performed on a subset of the same individuals using only one primer combination. Factorial analysis of correspondences and cluster analysis of one primer combination. Factorial analysis of correspondences and cluster analysis of the polymorphic fragments, scored as dominant markers, revealed, independently for the nuclear and the cytoplasmic markers, a striking specific specific and geographical structuring. Moreover, an interesting feature is that the AFLP gave the same results as the nuclear RFLP in terms of genetic diversity analysis. This study reveals a strong structuring inseide E. oleifera, according to the geographical origin, and also a slight intraregional structure within the Brazilian-Amazonian origins of the material studied. aBiogeography aGenetic markers aGenetic variation aCaiaué aDendê aDistribuição Geográfica aElaeis Guineensis aElaeis Oleifera aMarcador Genético aVariação Genética aBarcella odora1 aSECOND, G.1 aKAHN, F.1 aAMBLARD, P.1 aLEBRUN, P.1 aSEGUIN, M. tIn: HENDERSON, A.; BORCHSENIUS, R. (Ed.). Evolution, variation, and classification of palms. Bronx: NYBG, 1999.