02255naa a2200301 a 450000100080000000500110000800800410001902400540006010000260011424501730014026000090031352013240032265000180164665000140166465000260167865000110170465000220171565000140173765000150175165000140176665000220178065000090180270000160181170000170182770000180184470000200186277300710188216633562022-05-17       2009    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.ejsobi.2009.07.0032DOI1 aFERREIRA, E. P. de B.  aAssessing insecticide and fungicide effects on the culturable soil bacterial community by analyses of variance of their DGGE fingerprinting data.h[electronic resource]  c2009  aTo assess the effects of three insecticides (aldicarb, chlorpyrifos, deltamethrin) and two fungicides (tebuconazole and metalaxylþmancozeb) on the PCR-DGGE fingerprints of culturable soil bacterialcommunities (CSBC), a greenhouse experiment was carried out with soil samples from an Integrated System for Agroecological Production (ISAP), a Conventional Potato Production Area (CPPA) and a Secondary Forest Area (SFA) close to the CPPA. Samples were obtained at 15 day intervals starting at 32 until 77 days after sowing (DAS) to perform the PCR-DGGE analysis of the CSBC cultured on media amended with soil suspension. Analysis of variance from PCR-DGGE data indicated significant differences among treatments. Regardless the type of pesticide applied, CSBCwas disturbed and similarity values varied from 5% to 90% in comparison to the control. Significant shifts on CSBC were only detected among treatments in the first two harvests, while CSBC tended to be more akin to each other at the last two harvest dates. The most significant responses observed were due to different soil sample origins, where values of 5% of similarity to the control were observed on CPPA soil. The use of analysis of variance on PCR-DGGE data was useful to a better understanding of the changes on CSBC induced by pesticides applications.  aSoil bacteria  aBactéria  aBacteriologia do solo  aBatata  aControle Químico  aFungicida  aInseticida  aPesticida  aSolanum Tuberosum  aSolo1 aDUSI, A. N.1 aCOSTA, J. R.1 aXAVIER, G. R.1 aRUMJANEK, N. G.  tEuropean Journal of Soil Biologygv. 45, n. 5/6, p. 466-472, 2009.