02297naa a2200277 a 450000100080000000500110000800800410001910000160006024500900007626000090016650001320017552013480030765000200165565000160167565300170169165300210170865300360172970000210176570000230178670000200180970000250182970000190185470000310187370000230190477300920192716624572018-06-25       2009    bl uuuu     u00u1 u    #d1 aCURI, R. A.  aGenetic polymorphisms related to meat traits in purebred and crossbred Nelore cattle.  c2009  aTítulo em português: Polimorfi smos genéticos relacionados às características da carne em bovinos Nelore puros e cruzados.  aThe objective of this work was to estimate the allelic and genotypic frequencies of CAST/XmnI, a calpastatin gene polymorphism, and CAPN530, a calpain 1 large subunit gene polymorphism, in different beef genetic groups (Nelore and Nelore x Bos taurus), and to investigate associations between these polymorphisms and carcass and meat traits. Three hundred animals ? comprising 114 Nelore, 67 Angus x Nelore, 44 Rubia Gallega x Nelore, 41 Canchim, 19 Brangus three-way cross and 15 Braunvieh three-way cross ? were genotyped by PCR-RFLP and phenotyped for rib-eye area (REA), back-fat thickness (BT), intramuscular fat (IF), shear force (SF) and myofibrillar fragmentation index (MFI). The occurrence of the two alleles of the CAST/XmnI and CAPN530 single nucleotide polymorphisms (SNPs) in a B. indicus breed, which permitted association studies in purebred and crossbred Nelore cattle, was first shown in the present work. No relationship was found between the CAST or CAPN1 SNPs and growth-related traits (REA) or fat deposition (BT and IF), since calpastatin and ?-calpain are not physiologically involved with these traits. Moreover, the association results between genotypes and aged meat tenderness (assessed by SF and MFI) showed that these markers are useless in assisted selection for purebred Nelore and their crosses with B. taurus.  aMeat tenderness  aBos Indicus  aCalpain gene  aCalpastatin gene  aSingle nucleotide polymorphisms1 aFORTES, M. R. S.1 aCHARDULO, L. A. L.1 aSILVEIRA, A. C.1 aDE BENI ARRIGONE, M.1 aMARTINS, C. L.1 aASSUMPÇÃO, M. E. O. D'A.1 aOLIVEIRA, H. N. de  tPesquisa Agropecuária Brasileira, Brasília, DFgv. 44, n. 12, p. 1660-1666, dez. 2009