01582naa a2200253 a 450000100080000000500110000800800410001902400350006010000180009524501470011326000090026052007910026965000260106065000230108665000280110965300260113770000170116370000220118070000150120270000170121770000180123470000170125277300590126916621572026-04-14 2009 bl uuuu u00u1 u #d7 a10.1007/s12686-009-9010-22DOI1 aSANTOS, M. O. aIsolation and characterization of microsatellite loci in the tropical forage legume Stylosanthes guianensis (Aubl.) Sw.h[electronic resource] c2009 aABSTRACT: Stylosanthes guianensis is an important tropical pasture legume. Knowledge of genetic diversity and structure od S. guianensis populations is of great importance for the conservation and germplasm management of this species. Thus, 20 microsatellite markers were developed from a S. guianensis enriched library. The microsatellites were characterized in 20 accessions from the germplasm collection of the Empresa Brasileira de Pesquisa Agropecuária (Embrapa). The average number of alleles per locus varied from 2 to 7, with an average of 4 alleles per locus. The observed and expected heterozygosities ranged from 0 to 0,60 and 0,10 to 0,85, respectively. This new set of microsatellites will contribute towards studies of genetic diversity and conservation od S. guianensis. aLeguminosa Forrageira aMarcador Molecular aStylosanthes Guianensis aDiversidade genética1 aKARIA, C. T.1 aRESENDE, R. M. S.1 aCHIARI, L.1 aJUNGMANN, L.1 aZUCCHI, M. I.1 aSOUZA, A. P. tConservation Genetics Resourcesgv. 1, p. 43-46, 2009.