02458nam a2200217 a 450000100080000000500110000800800410001910000190006024501340007926002120021330000140042550000150043952016660045465000110212065000110213170000160214270000230215870000190218170000200220070000200222016556352023-07-10       2008    bl uuuu     u00u1 u    #d1 aNOVELLI, V. M.  aNatural infection of peace lily (Spatiphyllum wallisi) by a nuclear type of Brevipalpus-transmitted virus.h[electronic resource]  aIn: INTERNATIONAL SCIENTIFIC SEMINAR ON PLANT HEALTH, 6.; LATIN AMERICAN AND CARIBBEAN SYMPOSIUM, 2., 2008, La Habana, Cuba. Acarina Biodiversity: their use, protection and conservation. Havana, [s.n.]c2008  c1 CD-ROM.  a(E.3.1.42)  aPeace lily also known as sail plant (Spathiphyllum wallisi Regel, Araceae) has its origin in South America and widely used as in house ornamental under tropical conditions. One among several potted plants acquired from a nursery at Cordeiropólis, SP, Brazil, developed intense chlorotic spots which later became necrotic, associated with infestation by the tenuipalpid mite Brevipalpus sp. Electron microscopic examination of thin sections from the chlorotic lesions revealed electron lucent inclusions in the nucleus and the presence of short, rod-like particles scattered within the nucleus or in the cytoplasm. These particles were commonly arranged perpendicularly with membranes of the endoplasmic reticulum and nuclear envelope. This type of cytopathology is the same caused by the nuclear type of the Brevipalpus-transmitted viuses (BTV). RT-PCR assays were made using primers that amplify genome of known nuclear type of BTV ( Orchid fleck virus - OFV, Coffee ringspot virus - CoRSV, Clerodendrum chlorotic spot virus - ClCSV). Consistent amplifications were obtained using primers for CoRSV but not for OFV and CoRSV. Amplicon sequencing and 2x2 camparisons showed 93% of similarity with CoRSV sequence. Mechanical and mite transmission failed the virus from S. wallisi to S. wallisi as well as to coffee and other assay plants. Though CoRSV is able to infect many assay plant under experimental conditions, natural infection has been confirmed only in some species of Coffee and Psylanthus ebracteolatus, a rubiacea plant related to the genus Coffee. If confirmed this will be the first case of natural infection of a plant by CoRSV out of rubiaceae.  aÁcaro  aVírus1 aKUBO, K. S.1 aFREITAS-ASTÚA, J.1 aMACHADO, M. A.1 aSALAROLI, R. B.1 aKITAJIMA, E. W.