02460nam a2200277 a 450000100080000000500110000800800410001910000290006024501030008926001570019250000080034952015930035765000100195065000190196065000090197965300130198870000250200170000170202670000210204370000180206470000230208270000180210570000240212370000160214770000190216316556312023-07-10       2008    bl uuuu     u00u1 u    #d1 aBOSCARIOL-CAMARGO, R. L.  aGene expression of Rangpur Lime (Citrus limonia Osbeck) under water stress.h[electronic resource]  aIn: THE INTERNATIONAL CONFERENCE ON THE STATUS OF PLANT & ANIMAL GENOME RESEARCH, 16., 2008, San Diego. Final abstracts guide. San Diego: Illuminac2008  aP24  aRangpur lime (Citrus limonia Osbeck), a drought-tolerant citrus rootstock, has been widely used by growers in Brazil. Over than 160 million orange trees in Brazil are grafted on Rangpur lime and do not need supplementary irrigation. The present study aims to evaluate transcriptional responses of Rangpur lime rootstock to osmotic stress, and, identify genes possibly involved in drought tolerance. Plantlets were exposed to stress conditions using PEG6000 in nutritional soluction. RNA from roots was obtained and generated a total of 4,130 valid cDNA reads, with 2,020 from the non-stressed condition and 2,110 from the stressed set. Bioinformatic analyses measured the frequency of each read in the libraries and yielded an in silico transcriptional profile for each condition. A total of 39 contigs were differentially expressed and allowed to detect up-regulated homologue sequences to well known genes involved in stress responses, such as aquaporins, dehydrin, sucrose synthase, and proline-related synthase. Some sequences could not be classified by using FunCat and remained with an unknown function. A large number of sequences presented high similarities to annotated genes involved with cell energy, protein synthesis and cellular transport, suggesting that Rangpur lime may sustain active cell growth under stressed condition. The presence of membrane transporters and cell signaling components could be an indication of a coordinated morphological adaptation and biochemical response during drought, helping to explain the higher tolerance of this rootstock to water stress.  aÁgua  aFruta Cítrica  aGene  aEstresse1 aSILVA-PINHATI, A. C.1 aSOUZA, A. A.1 aAMARAL, A. M. do1 aCARLOS, E. F.1 aFREITAS-ASTÚA, J.1 aTAKITA, M. A.1 aTARGON, M. L. P. N.1 aREIS, S. M.1 aMACHADO, M. A.