01515nam a2200205 a 450000100080000000500110000800800410001910000200006024502090008026000750028952007850036465000220114965300110117170000210118270000250120370000190122870000180124770000230126570000210128816556212023-06-30       2008    bl uuuu     u00u1 u    #d1 aKITAJIMA, E. W.  aIn situ detection of CiLV-C in the mite vector Brevipalpus phoenicis, evidence for a circulative type of virus/vector relationship and a model for the virus circulation in the mite.h[electronic resource]  aTropical Plant Pathology, Brasilia, DF, v. 33, supl., ago. 2008.c2008  aPresumed CiLV-C particles have been observed between membranes of adjacent cells near midgut and prosomal glands of viruliferous Brevipalpus phoenicis (Acari:Tenuipalpidae). Their viral nature was demonstrated by in situ immunogold labeling with a specific polyclonal antibody raised against CiLV-C p29, putative capsid protein, expressed in E. coli. No evidence for viral replication as the presence of viroplasm was obtained suggesting that the CiLV-C/ B. phoenicis relationship is of a circulative type in agreement with transmission and qRT-PCR data. Based on the intercellular localization of the CiLV-C virions, a model for their circulation within B. phoenicis is proposed, similar to that of the paracellular route of leukocyte diapedesis through the capillary endothelium  aDoença de Planta  aVirose1 aCALEGARIO, R. F.1 aLOCALI-FABRIS, E. C.1 aNOVELLI, V. M.1 aBASTIANEL, M.1 aFREITAS-ASTÚA, J.1 aFRANCISCHINI, F.