02472naa a2200385 a 450000100080000000500110000800800410001902400420006010000160010224501360011826000090025452014020026365000220166565000110168765000110169865000150170965000230172465000140174765000170176165300180177865300140179665300210181065300200183165300150185165300200186665300120188670000150189870000150191370000180192870000160194670000180196270000180198070000170199877300710201516554142023-06-29       2009    bl uuuu     u00u1 u    #d7 a10.1111/j.1365-3180.2009.00746.x2DOI1 aDITA, M. A.  aGene expression profiling of Medicago truncatula roots in response to the parasitic plant Orobanche crenata.h[electronic resource]  c2009  aOrobanche crenata is a root parasitic weed that is a major constraint for grain and forage legume cultivation in Mediterranean and West Asia. Only moderate to low levels of incomplete resistance of complex inheritance has been identified so far in legume crops, which has hampered genetic and genomic analysis. In the present study, we provide a gene expression profile of roots of the model legume Medicago truncatula in response to infection by O. crenata. M. truncatula accessions SA27774 (complete resistance acting at early penetration stages) and SA4087 (incomplete late acting resistance mediated by necrosis of parasite tubercle) were inoculated with O. crenata seeds in a semi-sterile dish system. Roots were harvested at 15 (first contacts of the parasitism structures with the host roots), 21 (initial stage of parasite tubercle formation on SA4087) and 35 (prior necrosis of well-developed parasite tubercle of on SA4087) days post-inoculation. Array hybridisations revealed several hundred genes up-regulated in response to O. crenata infection. Gene expression patterns suggest that resistance mechanisms activated in both genotypes are temporally and spatially different and resemble those associated with plant resistance to microbial pathogens. Regulated genes identified here represent a comprehensive resource that can be used as a support to breeding strategies for resistance.  aDoença de Planta  aGenoma  aLegume  aLeguminosa  aNutrição Vegetal  aPatógeno  aResistência  aBarrel medick  aBroomrape  aDefence response  aGene regulation  aMicroarray  aParasitic plant  aProtein1 aDIE, J. V.1 aROMÁN, B.1 aKRAJINSKI, F.1 aKÜSTER, H.1 aMORENO, M. T.1 aCUBERO, J. I.1 aRUBIALES, D.  tWeed Research, Oxfordgv. 49, n. 1, p. 66-80, Oct. 2009. Suppl. 1.