02505naa a2200349 a 450000100080000000500110000800800410001902200140006010000200007424501290009426000090022352015140023265000170174665000150176365000110177865000260178965000220181565000110183765300170184865300140186565300170187970000160189670000260191270000250193870000170196370000170198070000230199770000220202070000210204270000200206377300720208316549882009-01-09       2008    bl uuuu     u00u1 u    #d  a0103-90161 aKITAJIMA, E. W.  aChlorotic spots on Clerodendrum, a disease caused by a nuclear type of Brevipalpus (AcaribTenuipalpidae) transmitted virus.  c2008  aChlorotic spots have been observed in plants of Clerodendrum x speciosum growing in residential gardens and parks in Piracicaba, SP, Brazil. Thin sections of diseased tissues revealed characteristic cytopathic effects of the nuclear type of the Brevipalpus (Acari: Tenuipalpidae) mite-transmitted viruses (BTrV). Brevipalpus mites, identified as B. phoenicis, infesting symptomatic C. x speciosum plants transmitted the pathogen to healthy C. x speciosum and to C. thomsonae, Gomphrena globosa, Hibiscus cannabinus, H. coccineus, H. schizopetalus, Salvia leucantha, Spathiphyllum wallasi and Tetragonia expansa causing chlorotic spots on their leaves. Mechanical inoculation using leaf extracts from infected C. x speciosum resulted in chlorotic spots on inoculated C. x speciosum, Chenopodium quinoa, C. amaranticolor, G. globosa, H. cannabinus, H. coccineus and T. expansa leaves. C. amaranticolor and C. quinoa kept at 28 - 30°C became systemically infected. The same cytopathic effects caused by the nuclear type of BTrV were seen in tissues from all infected test plants by electron microscopy. The virus was purified from systemically infected leaves of C. amaranticolor and C. quinoa. A polyclonal antiserum obtained from an immunized rabbit presented a strong reaction with the homologous antigen in ELISA tests. The results suggest that this chlorotic spot disease of C. x speciosum is caused by a new species of the nuclear type of BTrV, tentatively named Clerodendrum chlorotic spot virus (ClCSV).  aClerodendrum  ahost range  aÁcaro  aBrevipalpus Phoenicis  aDoença de Planta  aVírus  aPurification  aSpeciosum  aTransmission1 aKUBO, K. S.1 aFERREIRA, P. de T. O.1 aALCÂNTARA, B. K. de1 aBOARI, A. J.1 aGOMES, R. T.1 aFREITAS-ÁSTUA, J.1 aREZENDE, J. A. M.1 aMORAIS, G. J. de1 aSALAROLI, R. B.  tScientia Agricola, Piracicabagv. 6, n. 1, p. 36-49, jan/fev. 2008.