01504naa a2200145 a 450000100080000000500110000800800410001910000110006024500710007126000090014252010940015170000120124570000200125777300810127716519502004-04-20       1998    bl uuuu     u00u1 u    #d1 aLI, H.  aIsolation and characterisation of an acc oxidase genes in cassava.  c1998  aAbstract: Ethylene is involved in fruit ripening, wounding responses and other stress responeses. ACC oxidase catalyzes the last reaction of ethylene biosynthesis and is the key enzyme in the pathway. Many ACC oxidase genes have been characterized in other plant systems. During the post-harvest physiological deterioration (PPD) of cassava tuber ethylene production was found to increase after a lag of 6to 16 hours. The role of ethylene in the development of PPD is not clear, though there were some indications that ethylene is not directly involved in the process. To further understand the role of ethylene in development of PPD, na ACC oxidase cDNA was isolated from a PPD-related cDNA library using tomato ACC oxidase cDNA as probe. The 1085bp cDNA, designated as cACO1, showed  high similarity to cDNA sequences of Prunus persica, Pelargonium hortorum, Nicotiana tabacum and Solanum lycopersicum. A genomic clone was also isolated from a cassava genomic library (a gift from Professor M.A. Hughes) using cACO1 as a probe. Characterization of the ACC oxidase gene will be discussed.1 aHAN, Y.1 aBEECHING, J. R.  tRevista Brasileira de Mandioca, Salvadorgv.17, p.87, nov, 1998. Suplemento.