01514naa a2200253 a 450000100080000000500110000800800410001910000150006024501520007526000090022752007720023665000180100865000120102665000220103865000220106065300250108265300190110765300220112670000150114870000140116370000200117770000160119777300470121316503542023-05-10       2000    bl uuuu     u00u1 u    #d1 aSARRIA, R.  aTransgenic plants of cassava (Manihot esculenta) with resistance to Basta obtained by Agrobacterium-mediated transformation.h[electronic resource]  c2000  aTransgenic plants of cassava (Manihot esculenta) resistant to the herbicide Basta were obtained through Agrobacterium-mediated transformation. The plants also expressed the uidA gene and two were positive forPCR- and/or Sourthern-based detection of the nptII gene. Somatic-embryo-derived cotyledons were used as source of explants. A non-disarmed Agrobacterium strain (CIAT 1182) was used to transfer the genes of interest into cassava cultivar MPer183. Greenhouse tests of resistance to Basta (Hoechst) showed three plant lines with different levels of tolerance to the herbicide. Based on Southern tests of transgenesis, the transformation efficiency was 1%. The results constitute the first report of the bar gene conferring herbicide resistance to cassava plants.  aAgrobacterium  acassava  atransgenic plants  aManihot Esculenta  appt/Basta-resistence  atransformation  aUseful transgenes1 aTORRES, E.1 aANGEL, F.1 aCHAVARRIAGA, P.1 aROCA, W. M.  tPlant Cell Reportsgv.19, p.339-344, 2000.