01273naa a2200157 a 450000100080000000500110000800800410001910000180006024501270007826000090020552007970021465000220101170000210103370000150105477300460106916501722023-04-17       1995    bl uuuu     u00u1 u    #d1 aMYCOCK, D. J.  aCryoopreservation of somatic embryos of four species with anf without cryoprotectant pre-treatment.h[electronic resource]  c1995  aThe development of cryostorage for procedures for somatic embryos produced from the tissues of plants that normally propragate by means of desiccation-and (often) chilling-sensitive seeds, and that are unstorable by conventionalmeans offers viable alternative to the conservation of this otgerwiserecalcitrnat germplasm. A cryopreservation procedure utilizing cryoprotectants and partial dehydration was previously developed for hydrated and germinating Pisum sativum embryonic xes. The present contribuition aplies tjat technology to the somatic embryos of a range of species, viz., Coffea arabica (coffee), Maninhot esculenta cassava), Phoenix dactylifera 9date palm) and Pisum sativum (pea) and compares it withresults for material that was partially dehydrated, then very rapidily frozen.  aCriopreservação1 aWESLEY-SMITH, J.1 aBERJAK, P.  tAnnals of Botany.gv.75, p.331-336, 1995.