01240naa a2200181 a 450000100080000000500110000800800410001910000270006024500910008726000090017852007410018765000130092870000140094170000180095570000170097370000210099077300470101116501712023-04-17       1993    bl uuuu     u00u1 u    #d1 aRAEMAKERS, C. J. J. M.  aCyclic somatic embryogenesis and plant regeneration in cassava.h[electronic resource]  c1993  aWith a four-step procedure, using the cassava clone M. Col 22, a cyclic system of somatic embryogenesis was developed. Primary embryos were induced in step 1 (2, 4-D dependent) on yong leaf lobe explants. Primary embryos were isolated in step 2 (BAP dependent) and were used as starting for secondary embryogenesis (steps 1 and 2 repeated). In this way, repetitive embryogenic cultures were established. It was shown that in all sucessive cycles embryo explats produced significantly more embryos than leaf lobe explants. According to their shape embryos were classified in two development stages; torpedoshaped embryos and germinated embryos. Germinated embryos produe more than three times as many embryos than torpedo shaped embryos.  aMandioca1 aAMATI, M.1 aSTARITSKY, G.1 aJACOBSEN, E.1 aVISSER, R, G, F.  tAnnals of Botany.gv.71, p. 289-294, 1993.