01320naa a2200253 a 450000100080000000500110000800800410001910000140006024501070007426000090018152006260019065000180081665000180083465000190085265000180087165000110088965000180090065300230091865300230094165300200096465300100098470000200099477300520101416500652023-05-10       1989    bl uuuu     u00u1 u    #d1 aDIRKS, R.  aIn vitro plant regeneration from leaf and cotyledon explants of Cucumis melo L.h[electronic resource]  c1989  aFive different genotypes from in vitro as well as greenhouse grown melon plants were shown to be highly responsive for in vitro shoot formation from leaf explants when placed on basic MS medium supplemented with I mg/1 6-benzylaminopurine. In addition, a very suitable regeneration system was obtained when cotyledon pieces of mature seeds were incubated on the same culture medium. In this case, the first shoots already apperead after 10 days of incubation , and hundreds of shoots were from the cut surface 3 to 4 weeks later. Explants from mature cotyledons derived from seedlings did not lead to any shoot formation.  abiotechnology  aorganogenesis  atissue culture  aBiotecnologia  aMelão  aOrganogénese  aCultura de tecidos  aEfeito de genotipo  aGenotype effect  aMelon1 aBUGGENUM, M. V.  tPlant Cell Reports.gv.7, n.8, p.626-627, 1989.