01173naa a2200145 a 450000100080000000500110000800800410001910000190006024501900007926000090026952006360027865000140091470000170092877300820094516500162023-04-25       1976    bl uuuu     u00u1 u    #d1 aWILLIAM, E. B.  aNew approach to the cultivation of methanogenic bacteriab2-mercaptoethanesulfonic acid (HS-CoM)-Dependent growth of methanobacterium ruminatium in a pressurized.h[electronic resource]  c1976  aThe sensivity of the requirement of Methanobacterium ruminantium strain M1 to a new coenzyme, 2-mercaptoethanesulfonic acid (HS-CoM), was examined by use of new techniques that were developed for rapid and efficient handling of large numbers of cultures of methanogenic bacteria. The system uses sealed tubed that contain a gas mixture of 80% hydrogen and 20% carbon dioxide under a pressure of 2 to 3 atm. This modification of the Hungate technique reduces variability among replicate cultures and simplifies the dispensing, sterialization, and stoage of liquid media as well the transfer and maintenance of methanogenic bacteria.  aBactéria1 aWOLFE, R. S.  tApplied and Environmental Microbiology.gv.32, n.6, p.781-791, December-1976.