01533naa a2200145 a 450000100080000000500110000800800410001910000130006024501520007326000090022552010410023465000140127570000170128977300810130616499592023-04-25       1977    bl uuuu     u00u1 u    #d1 aCHEN, M.  aInfluence of CH4 production by Methanobacterium ruminant on the fermentation of glucose and lactate by Selenomonas ruminant.h[electronic resource]  c1977  aA method is described for increasing the production of H2 from glucose or lactate by Selenomas ruminatium by sequential transfers in media containing pregrown Methanobacterium ruminatium. The methanogen uses the H2 formed by the selenomonad to reduce CO2 to CH4. Analysis of fermetation products from glucose showed that lactate was the major product formed from glucose by S. ruminantium alone. Several sequential transfers in the presence of the methanogen caused a marked decrease in lactat production, which was accompanied by an increase in acetate. When lactate was the fermentation substrate, S.ruminantium alone produce propionate, acetate, and CO2. Addition to the pregrown methanogen in the sequential transfer procedure caused a significant decrease in the prodution of propionate and an increase in acetate formed from lactate. These results are interpreted in terms of the influence of H2 utilization by the methanogen on the production of H2 versus lactate or pripionate from reduced pyridine nucleotides by S. ruminatium.  aBactéria1 aWOLIN, M. J.  tApplied and Environmental Microbiologygv.34, n.6, p.756-759, December-1977.