01041naa a2200145 a 450000100080000000500110000800800410001910000170006024501020007726000090017952005930018865000160078170000170079777300810081416499122023-04-25       1977    bl uuuu     u00u1 u    #d1 aFERRY, J. G.  aNutritional and biochemical characterization of methanospirillum hungatii.h[electronic resource]  c1977  aTo ascertain its physiological similarity to other methanogenic bacteria, Methanospirillum hungatii, the type species of the genus, was characterized nutritionally and biochemically. Good growth occurred in a medium consisting of mineral salts, cysteine sulfide reducing buffer, and an H2-CO2 (80:20) atmosphere. Adittion of amino acids and B vitamins stimulated growth. Cellfree extracts contained methylcobalamin-coenzyme M methyltransferase, methylreductase, and formate hydrogenlyase. Cells contained coenzyme M and f420. Coenzyme F420 was required for formate hydrogenlyase activity.  aBioquímica1 aWOLFE, R. S.  tApplied and Environmental Microbiologygv.34, n.4, p.371-376, October- 1977.