01252naa a2200145 a 450000100080000000500110000800800410001910000190006024502250007926000090030452007030031365000110101670000160102777300630104316490672023-05-10       1989    bl uuuu     u00u1 u    #d1 aMANTELL, S. H.  aEffects of photoperiod, mineral medium strength, inorganic ammonium, sucrose and cytokinin on root, shoot and microtuber developmet in shoot cultures of dioscorea alata L. and D. bulbifera L. yams.h[electronic resource]  c1989  aThe effects of photoperiod (8, 12 or 16 h), mineral medium strength (dilutions of a tuberization medium, the T medium), sucrose (0,2,4,8%w/v) and kinetin (0,2.5 uM) on the development of roots, shoots and microtubers in shoot cultures of Dioscorea alata L. and D. bulbifera L. vams were evaluated. All of the factors were found to have substantial effects on microtuber induction in these two species. The effects of high and low inorganic ammonium containing media on microtuberization of yam shoot cultures indicated that ammonium ions inhibited microtuber induction in D. alata but not in D. bulbifera. Microtubers of D. alta were only formed on shoot cultures if these were held under 8-h days.  aInhame1 aHUGO, S. A.  tPlant Cell, Tissue and Organ Culturegv.16, p.23-37, 1989.