01550naa a2200265 a 450000100080000000500110000800800410001910000200006024500880008026000090016852008090017765000150098665000220100165000260102365000190104965000140106865300230108265300250110565300240113065300170115470000220117170000230119370000210121677300470123716484282023-05-15       1995    bl uuuu     u00u1 u    #d1 aDORNELAS, M. C.  aPlant regeneration from protoplast fusion in Passiflora spp.h[electronic resource]  c1995  aProtoplasts were isolated from leaf explants of Passiflora edulis var. flavicarpa (the yellow passion fruit) and from cell suspensions of five Passiflora species. Chemical fusion was performed using polyethylene glycol and the microcolonies obtained were transferred to growth medium to produce calli. Electrophoresis of soluble proteins and analysis of isoenzymes from calli produced from the fusion experiments were performed to select somatic hybrids. Specific polypeptide bands allowed the identification of somatic hybrids between P. edulis var. flavicarpa (+) P. alata, P. edulis var. flavicarpa (+) P. amethystina, P. edulis var. flavicarpa (+) P. cincinnata, P. edulis var. flavicarpa (+) P. giberti and P. edulis var. flavicarpa (+) P. coccinea. An average of 3 to 5% hybrid calli were obtained.  aPassiflora  aprotoplast fusion  asomatic hybridization  atissue culture  aMaracujá  aCultura de tecidos  aFusao de protoplasto  aHibridacao somatica  aPassionfruit1 aTAVARES, F. C. A.1 aOLIVEIRA, J. C. de1 aVIEIRA, M. L. C.  tPlant Cell Reportsgv.15, p.106-110, 1995.