01760naa a2200289 a 450000100080000000500110000800800410001910000160006024501340007626000090021052009350021965000180115465000190117265000140119165000220120565300170122765300230124465300170126765300260128465300210131070000170133170000200134870000210136870000170138970000170140677300470142316484272023-05-15       1994    bl uuuu     u00u1 u    #d1 aMANDERS, G.  aTransformation of passionfruit (Passiflora edulis fv flavicarpa Degener.) using Agrobacterium tumefaciens.h[electronic resource]  c1994  aLeaf and stem explants of passiofruit (Passiflora edulis fv flavicarpa) were co-cultivated with a disarmed strain of Agrobacterium tumefaciens harbouring the co-integrate vector pMON200. Four plants of passionfruit were regenerated from leaf explants on agar-sokidified Murashige and Skoog (1962) based medium containing 4,43 uM 6-benzyl-aminopurine and supplemented with 86 uM kanamycin sulphate. The four plants were rooted by transfer to MS based medium with 14,7 uM 3-indolebutyric acid and 2.68 uM a-naphthyleneacetic acid for 7 d, followed by MS based medium lacking growth regulators. Both media used for rooting contained 172 uM kanamycin sulphate. Rooted plants were potted and grown to maturity. Three of the plants synthesised nopaline and expressed neomycin phosphotransferase activity; DNA dot blot and polymerase chain reaction analyses confirmed the presence of the neomycin phosphotransferase gene in three plants.  aAgrobacterium  atissue culture  aMaracujá  aPassiflora Edulis  aAgrobacteria  aCultura de tecidos  aPassionfruit  aRegeneracao de apices  aShoot regeration1 aOTONI, W. C.1 aVAZ, F. B. d'U.1 aBLACKHALL, N. W.1 aPOWER, J. B.1 aDAVEY, M. R.  tPlant Cell Reportsgv.13, p.697-702, 1994.