01676naa a2200253 a 450000100080000000500110000800800410001910000160006024501330007626000090020952009230021865000210114165000190116265000190118165000220120065300230122265300160124565300180126165300220127965300200130165300190132170000190134077300630135916484112023-05-15       1987    bl uuuu     u00u1 u    #d1 aAMIN, M. N.  aRapid clonal propagation of guava through in vitro shoot proliferation on nodal explants of mature trees.h[electronic resource]  c1987  aIn vitro clonal propagation of guava `Banaras local' was achieved by culturing nodal explants of mature trees on Murashige and Skoog (MS) revised medium supplemented with 4.5 uM 6-benzyladanine (BA) alone or in combination with either 0.6 uM indole-3-acetic acid (IAA), 0.5 uM indole-3-butyric acid (IBA) or 0.3 uM gibberellic acid (GA3). Multiple shoots were induced to form by enhancement of axillary branching and BA (4.5 uM) without any auxin and gibberellin was found to give best shoot multiplication rate. In this medium 3-6 shoots developed on explants collected from field-grown plants and 5-10 shoots developed on explants taken from in vitro proliferated shoots within 12 wk of culture. a prior transfer of shoot clumps to a medium containing a lower concentration of BA (0.5 uM) before harvesting of cuttings for rooting allowed rapid extension growth and increased the number of usable shoots per culture.  amicropropagation  atissue culture  aFruta Tropical  aMicropropagação  aCultura de tecidos  aGema axilar  aNodal explant  aPsidium guajava L  aPsidium guava L  aTropical fruit1 aJAISWAL, V. S.  tPlant Cell, Tissue and Organ Culturegv.9, p.235-243, 1987