01621naa a2200241 a 450000100080000000500110000800800410001910000160006024500990007626000090017552009550018465000210113965000090116065000110116965000220118065300280120265300090123065300230123965300140126270000210127670000170129777300650131416484092023-05-15       1989    bl uuuu     u00u1 u    #d1 aDREW, R. A.  aThe transmission of banana bunchy-top virus in micropropagated bananas.h[electronic resource]  c1989  aPlants were established in vitro from banana bunchy-top virus (BBTV) infected plants. Explants containing either vegetative shoot apices or terminal floral apices were used to initiate cultures. Plants multiplied in culture were indistinguishable from nom-infected (control) plants and lacked characteristic symptoms of BBTV infection. After 16 months in culture plants were established in the glasshouse and after 1 month pots some plants started to show symptoms of the disease. After a further 5 months, 73% of the plants showed characteristic symptoms of the disease while 27% were symptomless and similar in appearance to control plants. These plants have been grown to maturity in the field without showing recognizable symptoms. This study demonstrates that BBTV can be transmitted in an appparently symptomless condition in culture and has important consequences for the dissemination of banana germplasm within Australia and internationally.  amicropropagation  aMusa  aBanana  aMicropropagação  aBanana bunchy-top virus  aBBTV  aCultura de tecidos  aTop virus1 aMOISANDER, J. A.1 aSMITH, M. K.  tPlant Cell, Tissue and Organ Culturegv.16, p.187-193, 1989.