01258naa a2200229 a 450000100080000000500110000800800410001910000170006024501320007726000090020952005990021865000110081765000190082865000220084765000070086965300110087665300110088765300220089865300230092065300190094377300660096216483852023-05-16       1994    bl uuuu     u00u1 u    #d1 aNIEDZ, R. P.  aGrowth of embryogenic sweet orange callus on media varying in the ratio of nitrate to ammonium nitrogen.h[electronic resource]  c1994  aEmbryogenic callus from Citrus sinensis (L.) Osbeck cv. Hamlin was cultured for 28 days on 20 media arranged in a 5 x 2 x 2 factorial varying in the ratio of nitrate to ammonium nitrogen, total inorganic nitrogen, and benzyladenine. Fresh weight increase of callus and final medium pH were significantly afffected by total inorganic nitrogen and the ratio of nitrate to ammonium. The nitrate to ammonium ratio accounted for 55% of the variation in the fresh weight increase of the callus and 93% of the variation in the final medium pH. Varying the NO3-:NH4+ ratio provided adequate pH control.  aCitrus  atissue culture  aCultura de Tecido  aPh  aBuffer  aCitros  aColo embriogenico  aEmbriogenic callus  aSolucao tampao  tPlant Cell, Tissue and Organ Culturegv.39, n.1, p.1-5, 1994.