01100naa a2200169 a 450000100080000000500110000800800410001910000140006024500830007426000090015752006460016670000150081270000170082770000120084470000180085677300560087416483361999-10-13       1996    bl ---       0--  u    #d1 aMAJER, D.  aThe use of AFLP fingerprinting for the detection of genetic variation in fungi  c1996  aA new PCR-based technique for the detection of inter-and intraspecific genetic variation has been tested on isolates of the fungal phytopathogens Cladosporium fulvum and Pyrenopeziza brassicae. The method is based on the selective PCR amplification of restriction fragments from digests of genomic DNA. We show that the technique is very efficient at detecting polymorphisms, even in species where very llittle variationcould previously be found by RFLP analysis. 21 primer combinations were used on four isolates of P. brassicae, detecting a total of 162 polymorphisms (mean = 4.I polymorphisms per primer combination per pair of isolates).1 aMITHEN, R.1 aLEWIS, B. G.1 aVOS, P.1 aOLIVER, R. P.  tMycological Researchgv.100, n.9, p.1107-1111, 1996