01345naa a2200193 a 450000100080000000500110000800800410001910000140006024500870007426000090016152008310017070000140100170000170101570000160103270000150104870000160106370000140107977300580109316483311999-10-11       1992    bl ---       0--  u    #d1 aYEL, S-D.  aComplete nucleotide sequence and genetic organization of papaya ringspot virus RNA  c1992  aThe complete nucleotide sequence of the RNA genome of papaya ringspot virus (PRSV) was determined from four overlapping cDNA clones and by direct sequenc ing of viral RNA. The genomic RNA is 10326 nucleotides in length, excluding the poly(A) tract, and contains one large open reading frame that starts at nucleotide positions 86 to 88 and ends at positions 10118 to 10120, encoding a polyprotein of 3344 amino acids. The highly conserved sequence at the 5' end of the RNA of PRSV and those of the other five reported potyviruses shows 80% similarity, suggesting that this region may play a common important role for potyvirus replication. Two cleavage sites of the polyprotein were determined by amido acid sequencing of the N termini of helper component (HC-Pro, amorphous inclusion) and cylindrical inclusion (CI) proteins.1 aJAN, F-J.1 aCHIANG, C-H.1 aDOONG, T-J.1 aCHEN, M-C.1 aCHUNG, P-H.1 aBAU, H-J.  tJournal of General Virologygv.73, p.2531-25541, 1992