01119naa a2200157 a 450000100080000000500110000800800410001910000190006024500470007926000090012652006780013565300260081365300300083970000190086977300730088816473201995-10-06 1969 bl --- 0-- u #d1 aCASTRIC, P. A. aCyanide metabolism by bacillus megaterium. c1969 aCyanide disappears from a medium supporting actively growing cells of Bacillus megaterium, a bacterium isolated from a cyanide enrichment medium containing Fargo clay soil. Whole cells of this organism convert K14CN to asparagine-14C, aspartic acid-14C and 14CO2. Besides cyanide, serine 14C ancd Beta-cyanoalanine-14C are effectively converted by whole cells of this organism to asparagine-14C. An analysis of asparagine isolated in an experiment in which whole cells were administered K13C15N and serine-14C shows that the nitrogen and carbon of the amide group is derived from cyanide. Beta-Cyanoalanine-14C was isolated and identified as a product of this experiment. aPreparacao de celular aPurificacao de asparagine1 aSTROBEL, G. A. tThe Journal of Biological Chemistrygv.244, n.15, p.4089-4094, 1969.