01199naa a2200169 a 450000100080000000500110000800800410001910000180006024501170007826000090019552006950020465000140089965300270091370000200094070000170096077300520097716471162023-04-12       1977    bl uuuu     u00u1 u    #d1 aDORSEY, T. E.  aA heated biuret-folin protein assay which gives equal absorbance with different proteins.h[electronic resource]  c1977  aThis protein assay requires heating the sample with biuret reagent for 100 min at 100grausC before addition of Folin. Different proteins give almost identical optical densities on a nitrogen basis with this procedure. The absorbance per microgram-atom of protein nitrogin is linear at or below 0.400. A convenient final concentration of protein in this assay is about 0.2 microg-atom of protein nitrogen per ml. Most of the absorbance is due to amino acid combinations other than tyrosine and tryptophan in the protein. The specificity appears similar to that of the biuret where few compounds of biological importance interfere significantly in the assay at normal cellular concentrations.  aProteína  aBioquimical Nitrogenio1 aMcDONALD, P. W.1 aROELS, O. A.  tAnalytical Biochemistrygv.78, p.156-164, 1977.