01256naa a2200205 a 450000100080000000500110000800800410001910000170006024501580007726000090023552006080024465000210085265000200087365000200089365300190091365300200093265300160095270000190096877300630098716469042023-05-17       1988    bl uuuu     u00u1 u    #d1 aMARIN, M. L.  aSurvival of somatic embryos and recovery of plants of sweet orange (Citrus sinensis (L.) Obs.) after immersion in liquid nitrogen.h[electronic resource]  c1988  aSomatic embryos of `Washington Navel' sweet orange (Citrus sinensis (L.) Obs.)derived from in vitro cultured ovules excised from immature fruits, were frozen to the temperature of liquid nitrogen. A method of slow cooling at a rate of 0.5grausC min-1 down to-42grausc followed by storage in liquid nitrogen was used. Thawing was achieved by keeping the specimens at room temperature for 15 min. A small number of frozen embryos survived and developed into proliferating cultures that produced whole plants. The plants. obtained from frozen cultures were transferred to soil and are growing successfully.  acryopreservation  asomatic embryos  aCitrus Sinensis  aCryoprotection  aLiquid nitrogen  aPseudobuldi1 aDURAN-VILA, N.  tPlant Cell, Tissue and Organ Culturegv.14, p.51-57, 1988.