01754naa a2200193 a 450000100080000000500110000800800410001910000170006024500940007726000090017152011840018065000120136465000190137665000220139565300230141765300360144070000190147677300650149516468002023-05-17       1987    bl uuuu     u00u1 u    #d1 aSTAMP, J. A.  aSecondary somatic embryogenesis and plant regeneration in cassava.h[electronic resource]  c1987  aSomatic embryos isolated from mature seed-derived cotyledon cultures of cassava (Mannihot esculenta Crantz) underwent direct secondary somatic embryogenesis or plant development under appropriate incutation conditions. Isolated somatic embryos were subjected to a two-stage culture procedure similar to that which induced their development on cotyledon explants. This involved incubation for 24-30 days on Murashige and Skoog basal medium supplemented with 2-8 mgl-1 2,4-dichlorophenoxyacetic acid (2,4-D) (Stage I medium) before transfer to medium supplemented with 0.01 mgl-1 2,4-D and 0.1 mgl-1 6-benzylamino purine (BAP) (Stage II medium). Under these conditions, secondary somatic embryos developed directly from the cotyledons and shoot-tip region of primaty somatic embryos by a developmental process morphologically very similar to that occurring on zygotic cotyledon explants. Apical shoot extension and adventitious root formation occurred when somatic embryos were isolated from parental cultures and incubated on Stage II medium. Somatic embryo-derived plants growing in greenhouse conditions appeared morphologically normal when compared with non-regenerated plants.  acassava  atissue culture  aManihot Esculenta  aPlant regeneration  aSecondary somatic embryogenesis1 aHENSHAW, G. G.  tPlant Cell, Tissue and Organ Culturegv.10, p.227-233, 1987.