01434naa a2200205 a 450000100080000000500110000800800410001910000160006024501240007626000090020052008240020965000210103365000220105465300260107665300240110265300160112665300200114270000140116277300520117616467562023-05-17       1987    bl uuuu     u00u1 u    #d1 aOHNISHI, J.  aLight-Dependent Uptake of Pyruvate by Mesophyll Chloroplasts of a C4 Plant, Panicum miliaceum L.h[electronic resource]  c1987  aLighe-dependent active uptake of pyruvate was reported in mesophyll choloroplast of a C4 plant, Panicum miliaceum [Ohnishi and Kanai (1987) Plant Cell Physiol. 28: 1]. The present study tried to clarify the energy source of this active uptake. Preillumination of the mesophyll chloroplasts increased over tenfold their pyruvate uptake in the light and dark. This indicates that light itself is not essential for the enhancement. The pyruvate uptake capacity (the initial uptake rate) of the mesophyll chloroplasts increased on illumonation and reached a steady-state level after a few minutes; this rise sas faster under higher light intensities. When the chloroplasts were returned to darkness, theuptake capacity decayed with a half-life of about 1 min; this was independent of the light intensity of preillumination.  aactive transport  aC4 photosynthesis  aMesophyll chloroplast  aPanicum miliaceum L  apH gradient  aPyruvate uptake1 aKANAI, R.  tPlant Cell Physiol.gv.28, n.2, p.243-251, 1987