01447naa a2200205 a 450000100080000000500110000800800410001910000160006024500820007626000090015852008810016765000210104865000150106965000190108465300290110365300110113270000220114370000130116577300630117816467322023-05-17       1990    bl uuuu     u00u1 u    #d1 aREUVENI, O.  aIn Vitro Clonal Propagation of Diocious Carica Papaya.h[electronic resource]  c1990  aA procedure for in vitro propagation of dioecious papaya clones is described. A high rate of sucess in culture estbalishment was obtained when axiliary buds were taken from lateral shoots of hedged rooted cuttings grown in a greenhouse. Seasonal endophytic contamination was suppressed by shaking propagules for 24 h in 300 mgl rifampicin or by incorporating it at 50 mgl into the medium. Murashige & Skoog (MS) basal medium supplemented with 0.5 mgl 6-benzyladenine and 0.1mgl adenine sulfate improved multiplication and shoot growth. An elongation stage on MS medium supplemented with 1.0 mgl kinetin and 0.05 mgl naphthaleneacetic acid was necessary before rooting. Rooting was obtained at a high rate on half-strength macroelements of MS medium supplemented with 1.0mgl indole-3-butyric acid. Commercial plots of papya plants obtained through this procedure already exist.  amicropropagation  arifampicin  atissue culture  aEndophytic contamination  aPapaya1 aSHLESINGER, D. R.1 aLAVI, U.  tPlant Cell, Tissue and Organ Culturegv.20, p.41-46, 1990.