01761naa a2200277 a 450000100080000000500110000800800410001910000220006024501290008226000090021152009730022065000200119365000210121365000170123465000140125165000180126565000220128365300220130565300140132765300200134165300190136165300130138070000190139370000160141277300550142816466502023-05-30       1969    bl uuuu     u00u1 u    #d1 aROISTACHER, C. M.  aCitrus exocortis virus--chemical inactivation on tools, tolerance to heat and separation of isolates.h[electronic resource]  c1969  aExocortis virus was readily transmitted by contaminated knife blades and fingers. Mechanical transmission by knife cuts was prevented by a 1-second dip of the contaminated blades in diluted household bleach or a solution of 2% sodium hydroxide plus 2% formalin. Heating contaminated blades for 4 to 6 seconds in the flame of a propane torch or flaming blades dipped in 95% alcohol failed to inactivate the virus. A 1-second dip in the following chemical suspensions or solutions also failed to prevent transmission: 20% phisohex, 3% formalin, 2% trisodium phosphate cleaner, 10% borax, 1% lysol and 95% ethyl alcohol. a 20% household bleach solution (containing about 1% sodium hypocrlorite) is recommended as a disinfectant for pruning and grafting tool. The corrosive action of the bleach on tools was avoided by dipping in a mixture of vibegar, water, and emulsifiable oil. Mild isolates of exocortis virus were separated from severe isolates by knife transmission.  aCitrus jambhiri  aCitrus latifolia  aCitrus limon  aBactéria  aCitrus Medica  aCitrus Reticulata  aCasa de vegetacao  aCitrus ap  aCitrus paradesi  aCitrus sinesis  aControle1 aCALAVAN, E. C.1 aBLUE, R. L.  tPlant Disease Reportergv.53, n.5, p.333-336, 1969