01308naa a2200181 a 450000100080000000500110000800800410001910000200006024501220008026000090020252007720021165300270098365300080101065300190101865300140103770000180105177300570106916466411995-10-10       1967    bl ---       0--  u    #d1 aHARDY, R. W. F.  aAtp-dependent reduction of azide and HCN by N2-fixing enzymes of azotobacter vinelandii and clostridium pasteurianum.  c1967  aThe novel reductions of HCN to CH4, NH3 and tentatively, small amounts of CH3NH2, and of N3- to NH3 and N2 are demostrated with cell-free extracts of Azotobacter vinclandii and Clostridium pasteurianum. Requirements for the reductions are: (i) extracts of cells grown on N2 (those grown on urea or NH3 are infective); (ii) ATP-generator; and (iii) reductant (Na2S2O4 or in some cases H2 or KBH4) and characteristics of the reductions are: (i) inhibition by either substrate of ATP-dependent H2 evolution from Na2S2O4, but no effect on reductant-dependent ATPase; and (ii) complete inhibition by CO. The rate of electron consumption for reduction of N3- is similar to the rate for reduction of N2 or N2O, while the rate for reduction of HCN is about 30% of the others.  aCrescimento de celulas  aHCN  aMicrorganismos  aReagentes1 aKNIGHT Jr. E.  tBiochimica et Biophysica Actagv.139, p.69-90, 1967.