01784naa a2200181 a 450000100080000000500110000800800410001910000180006024501830007826000090026152011710027065000130144165300220145465300260147670000170150270000180151977300650153716464502023-05-17       1993    bl uuuu     u00u1 u    #d1 aJOSEPH, C. V.  aGlycerol stimulation of chlorophyll synthesis, embryogenesis, and carboxylation and sucrose metabolism enzymes in nucellar callus of 'Hamlin' sweet orange.h[electronic resource]  c1993  aPhosphoenolpyruvate carboxylase (PEPCase) and sucrose phosphat synthase (SPS) were active in nucellar calli of 'Hamilin' sweet orange, Citrus sinensis (L.) Osbeck, grown on media containning either 5% sucrose or 2% glycerol as the primary carbon source. Activities of the enzymes,however, were much higher in the glycerol-grown tissues than those grown in sucrose. Glycerol, in addition, stimulated embryogenesis and chlorophyll biosynthesis and initiated the onset of ribulose bisphosphatecarboxylase-oxygenase (Rubisco) activity. These stimulatory effects, which were not observed in sucrose-grown calli, became more obvious when calli grown on glycerol-containing, agar-solidified medium were transferred and grown in liquid suspension containing 2% glycerol. Starchlevels in both sucrose and glycerol-grown tissues were as high as 30% of tissue dry weight. There were, however, higher concentrations of soluble sugars in sucrose-grown calli than those grown on glycerol. Stimulation of embryogenesis, chlorophyll synthesis, Rubisco onset, and activities of PEPCase and SPS by glycerol offered potential prospects in using this compound in citrus tissue cultures.  aglycerol  aCarbon metebolism  aCitrus tissue culture1 aNIEDZ, R. P.1 aYELENOSKY, G.  tPlant Cell, Tissue and Organ Culturegv. 33, p. 75-80, 1993.