01669naa a2200253 a 450000100080000000500110000800800410001902000180006010000120007824500890009026000090017930000140018850002560020252007390045865000190119765000130121665300160122965300280124565300260127370000110129970000180131070000200132877300670134816458812000-05-24       2000    bl uuuu     u00u1 u    #d  a85-87697-05-61 aHAN, Y.  aIsolation of post-harvest physiological deterioration related cDNA clones in cassava  c2000  ap.526-536  aIn: INTERNATIONAL SCIENTIFIC MEETING ON CASSAVA BIOTECHNOLOGY, 1998. Salvador-Bahia. Proceedings... Brasilia, DF: Embrapa Recursos Geneticos e Biotecnologia/CBN, 2000. 625p. Editado por Luiz J. C. B. Carvalho, Ann Marie Thro, Alberto Duarte Vilarinhos  aPost-harvest physiological deterioration (PPD) is a severe problem affecting the marketability and use of cassava storage roots. While PPD resembles wound responses studied in other plants, extensive wound healing is lacking. In an attempt to understand this phenomenon we have constructed a cDNA library from mRNA isolated 48 hours after harvesting, from which we have isolated and sequenced several clones for genes likely to be involved in the PPD response. These include: 1) a clone for a hydroxyproline-rich glycoprotein (HRGP) - HRGPs are structural proteins of the plant cell wall which are involved in barrier formation during wound healing and defence against pathogens; 2) a clone for B-1,3-glucanase-an enzyme which breaks.  aplant breeding  aMandioca  aArmazenagem  aFisiologia pos-colheita  aMelhoramento genetico1 aLI, H.1 aCOOPER, R. M.1 aBEECHING, J. R.  tBrasilia: Embrapa Recursos Geneticos e Biotecnologia/CBN, 2000