01565naa a2200301 a 450000100080000000500110000800800410001902200140006010000240007424500800009826000090017852007110018765000180089865000180091665000180093465000180095265000200097065000170099065000180100765300170102565300270104270000180106970000190108770000170110670000280112370000200115177300920117116390052009-01-09       2008    bl uuuu     u00u1 u    #d  a0100-204X1 aMENDES, A. F. da S.  aTissue culture parameters in sweet orange cultivars.h[electronic resource]  c2008  aThe objective of this work was to establish tissue culture parameters for gene transfer in sweet orange cultivars. Epicotyl explants with different ages were cultured with 6-benzylaminopurine (BAP), kanamycin and hygromycin. Shoots were cultured with alpha-naphthaleneacetic acid (NAA) alone or in combination with indole-3-butyric acid (IBA). The requirement of BAP for shoot development was genotype-specific. Epicotyl explants from 35-day-old seedlings produced significantly more shoots per explant in 'Pêra'. Kanamycin inhibited shoot regeneration for the most cultivars. The percentage of shoots that produced roots in 'Pêra' was significantly higher in medium with NAA and IBA than with NAA alone.  abiotechnology  agene transfer  amorphogenesis  aBiotecnologia  aCitrus Sinensis  aMorfogênese  aRegeneração  aregeneration  atransferência gênica1 aCIDADE, L. C.1 aMANZOLI, G. N.1 aOTONI, W. C.1 aSOARES FILHO, W. dos S.1 aCOSTA, M. G. C.  tPesquisa Agropecuária Brasileira, Brasília, DFgv. 43, n. 8, p. 1093-1096, ago. 2008.