01681naa a2200289 a 450000100080000000500110000800800410001902200140006010000190007424501060009326000090019952009030020865000230111165000190113465000220115365000210117565300120119665300170120865300120122570000170123770000140125470000180126870000180128670000160130470000200132077300510134016357512000-06-09 1998 bl uuuu u00u1 u #d a0248-12941 aSIMONEB, M. De aConstruction of genetic maps for Citrus aurantium and C. latipes based on AFLP, RAPD and RFLP markers c1998 aCitrus have a word-wide economic importance. Their breeding is a long process complicated by a long juvenile period, heterozygosity and nucellar embryony. Molecular markers are an important tool for the identification of chromosomal regions carrying loci controlling important agronomic traits in citrus and for marker assisted rootstock breeding. Then, they were tested for the construction of linkage maps for C. aurantium and C. latipes. Polymorphism of 50 F1 individual of a single controlled cross between C. latipes and C. aurantium were carried out from the study of AFLP markers (7 AFLP EcoRI/Msel primer combinations), RAPD markers (280 primers) and the use of 50 RFLP probes, isolated from a Citrus PstI genomic library. were scored according to the pseudo-testcross strategy and two linkage maps - one for C. latipes and one for C. aurantium - were constructed using a suitable software. aanalytical methods aCitrus latipes amolecular biology aCitrus Aurantium aGenetic aGenetic maps aMarkers1 aRUSSO, M. P.1 aPULEO, G.1 aMARSAN, P. A.1 aLORENZONI, C.1 aMAROCCO, A.1 aRECUPERO, G. R. tFruitsgv.53, n.6 383-390, Paris, France, 1998