02028naa a2200349 a 450000100080000000500110000800800410001910000250006024501280008526000090021352010200022265000160124265000190125865000170127765000110129465300260130565300210133165300230135265300300137565300410140565300330144665300150147965300090149465300140150365300130151765300100153065300200154070000210156070000190158170000260160077300520162616342462023-05-17       1995    bl uuuu     u00u1 u    #d1 aMOHAMED-YASSSEEN, Y.  aIn vitro shoot proliferation ao propagation of guava (Psidium guajava L.) from germinated seedlings.h[electronic resource]  c1995  aGuava seeds were germinated on Murashige and Skoog (MS) medium with orwithout 8.8 uM benzyladenine (BA). BA increased the rate of germination and the number of lateral shoots (3,4 vs 1,2 per seedling). Stem nodes from these lateral shoots were cultured on proliferation media with 4,.4 uM BA, and multiple shoots (3.5) were formed within 4 weeks of culture. Increasing the concentration of BA or the addition of naphthaleneacetic acid (NAA) did not affect shoot formation.Shoots produced from explants and lateral shoots from germinated sedlings were rooted in media containing activated charcoal (AC) or 9,8 uM indolebutyric acid (IBA). Shoots rooted with IBA had a higher rooting percentage (100% vs 75%) and a greater number of roots (5.5 vs 3.2) but the shoots were shorter (2.6 vs 3.4 cm) than when rooted in AC, and they required an additional 4 weeks of culture in media hith AC to achieve shoot elongation. About 80% of the shoots with roots survived in the glasshouse and produced normal phenotypic plants.  agermination  atissue culture  aGerminação  aGoiaba  aAC-activated charcoal  aBA-benzyladenine  acultura de tecidos  aIBA-indole-3-butyric acid  aMS-Murashige and Skoog (1962) medium  aNAA-1-naphthaleneacetic acid  aPropagacao  aSeed  aSelection  aSementes  aShoot  aTDZ-thidiazuron1 aBARRINGER, S. A.1 aSCHNELL, R. J.1 aSPLITTSTOESSER, W. E.  tPlant Cell Reportsgv.14, n.8, p.525-528, 1995.