01758naa a2200301 a 450000100080000000500110000800800410001910000230006024500700008326000090015352009920016265000130115465000160116765000170118365000140120065300230121465300120123765300080124965300290125765300120128665300080129865300260130665300140133265300110134665300270135770000200138477300520140416342272023-05-17       1995    bl uuuu     u00u1 u    #d1 aGARCIA-AGUSTIN, P.  aSelection of a NaCl-tolerant Citrus plant.h[electronic resource]  c1995  aThe objective of the work was to select Citrus plants more tolerant to elevated NaCl concentrations in the irrigation water. For this porpuse, unfertilized Troyer citrange ovules treated with a chemical mutagenic agent (ethyl methane sulphonate) were cultured in vitro. Whole plants were regenerated from embryos developed in the nucellar tissue of the ovule. The screening for salt tolerance was achieved by irrigating these plants with a nutrient solution containintg 45 mM NaCl. Plants obtained from vegetative propagation of the selected plant show faster growth, less leaf damage and lower concentrations of Cl- and Na+ in leaves that the original clone with increasing NaCl levels in the nutrient solution. In addition, the selected plant accumulated more Na+ inshoots and roots and this was accompanied by a significant reduction in K+ concentration. It can be concluded that the selected plant restricts the accumulation of Na+ in leaves by concentrating Na+ in shoots and roots.  abreeding  avariability  aMelhoramento  aSeleção  aCultura de tecidos  aCulture  aEMS  aEthyl-methane-sulphonate  aGenetic  aIBA  aIndole 3-butyric acid  aSelection  aTissue  aVariabilidade genetica1 aPRIMO-MILLO, E.  tPlant cell reportsgv.15, n.5, p.314-318, 1995.