02035naa a2200313 a 450000100080000000500110000800800410001910000170006024501090007726000090018630000120019552012280020765000140143565000120144965000200146165000110148165300090149265300190150165300120152065300190153265300180155165300240156965300080159365300160160170000170161770000150163470000200164977300520166916342191996-01-30       1995    bl uuuu     u00u1 u    #d1 aINOUE, A. K.  aCharacterization of passion fruit woodiness virus isolates from Brasilia and surrounding region, Brazil.  c1995  a479-487  aFour isolates of passion fruit woodiness virus (PWV) were collected from Passiflora edulis f. flavicarpa, P. edulis, P. alata  a and Phaseolus vulgaris in the Federal District and Cristalina, Goias State, and characterized. Cytoplasmic inclusions were seen at the ligh microscope which were later characterized as pinwheel and lamellar inclusions as seen in the electron microscope. Filamentous virus particles, typical of potyviruses, were seen in leaf dip preparations from infected samples. In many leaf parenchyma cells od infected plants some chloroplasts appeared in different degrees of disorganization. Serological reactions using antiserum against a Brazilian isolate of PWV resulted positive. The were inoculated onto several test plants, mostly leguminous,in an attemp to distinguish these isolates. Although some sligh differences were seen in the intensity of the symptoms, no distinction could be found among the isolates, They infected most cultivars of Phaseollus vulgar but not P. angularis, Vigna radiata and V. unguiculata. P. vulgaris cv. Rico-23, Rosinha and Preto-153 were the most susceptible with very severe symptoms; cv. Jalo was also infected, but cv. Top Cropand Dubbele Witte were not infected.  aPotyvirus  aDoença  aIdentificação  aVírus  aBean  aCaracterizacao  aMarcuja  aPassiflora spp  aPassion fruit  aPhaseollus vulgaris  aPWV  aTransmissao1 aMELLO, R. N.1 aNAGATA, T.1 aKITAJIMA, E. W.  tFitopatologia Brasileira, 20(3), setembro 1995.