01705naa a2200229 a 450000100080000000500110000800800410001902200140006010000200007424500790009426000090017352010770018265000260125965000180128565000210130365300170132465300160134170000160135770000160137370000160138977300700140516340852023-05-17       1995    bl uuuu     u00u1 u    #d  a0167-68571 aMONSALUD, M.-J.  aControl of hyperhydricity of mango somatic embryos.h[electronic resource]  c1995  aHyperhydricity of immature somatic embryos has been a limiting factor for the development of highly embryogenic suspension cultures of many important mango cultivars. Reversion of hyperhydricity was achieved in two ways: 1) heart-stage somatic embryos (2-3 mm length) were partially dehydrated under controlled conditions at high relative humidity (RH) for 24-48 h and 2) the gelling agent (Gel-Gro) concentration of theplant growth medium was increased from 2.0 to 6.0 g 1-1. Partially dehydrated immature somatic embryos were normal in appearance. Somatic embryos that were partially dehydrated germinated precociously when cultured on maturation medium. Although abscisic acid (ABA) did not reverse hyperhydricity of primary somatic embryos, ABA did stimulate the reversal of this abnormal pattern of development among secondary embryos.ABA (500 mM) inhibited precocious germination and permitted somatic embryo maturation. Partially dehydrated, immature somatic embryos (4-7 mm long) remained viable for up to 32 days in the absence of maturation medium under high RH.  asomatic embryogenesis  avitrification  aMangifera Indica  aAbscisc acid  aDehydration1 aMATHEWS, H.1 aLITZ, R. E.1 aGRAY, D. J.  tPlant Cell, Tissue and Organ Culturegv.42, n.2, p.195-206, 1995.