02092naa a2200169 a 450000100080000000500110000800800410001902200140006010000160007424501590009026000090024952015340025865000140179265300240180670000300183077300620186016331351995-04-19       1994    bl uuuu     u00u1 u    #d  a0331-949X1 aWANG, H. C.  aComparison of the Nuclear Inclusion b Protein and Coat Protein Genes of Five Papaya Ringspot Virus Strains Distinct in Geographic Origin andPathogenicity.  c1994  aA complementary DNA library was constructed to the genomic RNA of an isolate of papaya ringspot virus (PRSV), PRSV YK (YK), that represents the most prevalent mosaic strain of Taiwan. A 2.96-kb clone, pYKB9, corresponding to the 3'-region of YK RNA was selected by immunoscreening with the antiserum to the coat protein (CP). The clone had 2,960 nucleotide residuesand represented part of the nuclear inclusion a (NLa) gene, the complete nuclear inclusion b (NLb) gene, the entire CP gene, and the 3'-noncoding region of YK RNA. The nucleotide sequence was compared to those of the 3' regions of the published Hawaii severe strain, PRSV HA (HA), the Hawaii mild strain PRSV HA 5-1 (HA 5-1), the monpapaya-infecting Florida strain, PRSV W(fl) (W(fl), and the monpapaya-infecting Australia strain PRSV W(aust) (W(aust)). The NIb region had 83.1% nucleotide identity and 95.7% amino acid identity compared to thatof HA, with a total of 262 nucleotide differences resulting in only 22 amino acid changes. A similar trend also was found when the NIb region of YK was compared to the partially elucidated NIb regions of HA 5-1and PRSV W(fl), with 84.7 and 85.2% nucleotide identities (147 and 151 nucleotide differences), respectively: both resulting in 97.6% amino acid identity (only eight amino acid changes). Most of the nucleotide differences between the Asian and American strains occurred in wobble bases and, thus, conserved the amino acid residues, suggesting a common selection pressure to keep the same protein conformation.  aPotyvirus  aAmino acid sequence1 aBAU, J. H. and YEH, D. S.  tThe American Phytopathological Society vol.84 (10), 1994.