02363naa a2200253 a 450000100080000000500110000800800410001910000190006024501640007926000090024350001550025252014530040765000110186065300120187165300260188370000160190970000180192570000170194370000230196070000140198370000160199770000200201377300760203316294152015-03-18       2007    bl uuuu     u00u1 u    #d1 aHUMPHRY, D. R.  aPhylogenetic assignment and mechanism of action of a crop growth promoting Rhizobium radiobacter strain used as a biofertiliser on graminaceous crops in Russia  c2007  aParceria: University of York, University of Sunderland, Auburn University, University of Dundee, All-Russia Research Institute, Northumbria University  aThe taxonomic position of "Agrobac-terium radiobacter strain 204," used in Russia as a cereal crop growth promoting inoculant, was de-rived by a polyphasic approach. The phenotypic analyses gave very similar biochemical profiles for strain 204, Rhizobium radiobacter NCIMB 9042 (formerly the A. radiobacler type strain) and R. radiobacter NCIMB 13307 (formerly the Agrobacterium tumefaciens type strain). High percentage similarities, above the species separa-tion level, were observed between the 16S rRNA, fusA and rpoB housekeeping gene sequences of these three strains, and the genomic DNA-DNA hybridisation of strain 204 against the type strain of R. radiobacter NCIMB 9042 was over 70%. Strain 204 is not phytopathogenic and it does not fix atmospheric N2 or form a physical association with the roots of barley. Strain 204 culture and culiure supernatant stimulated the rate of mobilisation of seed reserves of barley in darkness and promoted its shoot growth in the light; Gibberellic acid (GA) concentration was 1.3 JlNI but indole acetic acid was undetectable ( &lt 50 nM) in cultures of strain 204. It is concluded that strain 204 is phenotypi-cally and genotypically very similar to the current R. radiobacter type strain and that the mechanism or its effect on growth or cereals is via the pro-duction of plant growth promoting substances. GA is likely to play an important role in the strain 204 stimulation or early growth or barley.  aCereal  aCereals  aRhizobium radiobacter1 aANDREWS, M.1 aSANTOS, S. R.1 aJAMES, E. K.1 aVINOGRADOVA, L. V.1 aPERIN, L.1 aREIS, V. M.1 aCUMMINGS, S. P.  tAntonie van Leeuwenhoek, Amsterdamgv. 91, n. 2, p. 105-113, feb. 2007.