02702naa a2200517 a 450000100080000000500110000800800410001910000160006024500880007626000090016452012820017365000180145565000170147365000210149065000160151165000080152765000140153565000140154965000160156365300280157965300330160765300080164065300250164865300210167365300260169465300260172065300080174665300400175465300110179465300150180565300120182065300150183270000260184770000240187370000140189770000170191170000140192870000170194270000220195970000150198170000190199670000190201570000170203470000260205177301070207716279982005-04-05       2004    bl ---       0--  u    #d1 aREIS, V. M.  aBurkholderia tropica sp. nov., a novel nitrogen-fixing, plant-associated bacterium.  c2004  aIn an ecological survey of nitrogen-fixing bacteria isolated from the rhizosphere and as endophytes of sugarce, maize and teosinte plants in Brazil, Mexico and South Africa, a new phylogenetically homogeneous group of N2-fixing bacteria was identified within the genus Burkholderia. This polyphasic taxonomic study included microscopic and colony morphology, API 20NE tests and growth on different culture media at different pH and temperatures, as well as carbon source assimilation tests and whole-cell protein pattern analysis. Analysis of 16S rRNA gene sequences showed 99.2-99.9% similarity within the novel species and 97.2% similarity to the closet related species, Burkholderia sacchari. The novel species was composed of four distinct amplified 16S rDNA restriction analysis groups. The DNA-DNA reassociation values within the novel species were greater than 70% and less than 42% for the closet related species, B. sacchari. Based on these results and on many phenotypic characteristics, a novel N2-fixing species is proposed for the genus Burkholderia, Burkholderia tropica sp. nov., with the type strain Ppe8T (=ATCC BAA-831T=DSM 15359T). B. tropica was isolated from plants grown in geographical regions with climates ranging from temperates subhumid to hot humid.  anucleic acids  asiderophores  aÁcido Nucléico  aCrescimento  aDNA  aFenótipo  aProteína  aSideróforo  aBactéria diazotrófica  aBiological nitrogen fixation  aBNF  aBurkholderia tropica  aCana-de-açúcar  aDeoxyribonucleic acid  aDiazotrophic bacteria  aFBN  aFixação biológica de nitrogênio  aGrowth  aPhenotypes  aProtein  aSugar cane1 aSANTOS, P. E. -de los1 aTENORIO-SALGADO, S.1 aVOGEL, J.1 aSTOFFELS, M.1 aGUYON, S.1 aMAVINGUI, P.1 aBALDANI, V. L. D.1 aSCHMID, M.1 aBALDANI, J. I.1 aBALANDREAU, J.1 aHARTMANN, A.1 aCABALLERO-MELLADO, J.  tInternational Journal of Systematic and Evolutionary Microbiology, Readinggv. 54, p. 2155-2162, 2004.